Finn Dennis Ledwidge, aged 21, of Manor Park, Onchan, and Dylan McGeown, aged 18, of River Heights, Douglas, this week pleaded not guilty to a joint charge of property damage.
A third man, Todd Crellin, aged 20, of Cronk y Berry, Douglas, entered a not guilty plea to the same charge on January 18.
A cottage at Groudle Glen was vandalised between December 31 and January 3.
Human faeces was found in a microwave oven, vomit on beds and a carpet, glass and a lamp were smashed, and doors were punctured with holes at the property.
They appeared in court this week when High Bailiff Jayne Hughes accepted summary court jurisdiction and a pre-trial review will be held for all three parties on March 22.
Bail continues in the sum of £500 for all three.